Trypanosoma brucei

This article is currently being developed as part of an Eduzendium student project. If you are not involved with this project, please refrain from collaboratively developing it until this notice is removed. Articles that lack this notice, including many Eduzendium ones, welcome your collaboration!

Classification

Higher order taxa

Kingdom: Protista
Phylum: Sarcomastigophora
Class: Zoomastigohora
Order: Kinetoplastida
Family: Trypanosomatidae

Species

Genus: Trypanosoma
Species: brucei
Trypanosoma brucei

Description and significance

[Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated. Include a picture or two (with sources) if you can find them.]

Trypanosoma brucei is a unicellular parasitic organism with no true tissues. It belongs to the protista kingdom and therefore has cell structures that are similar to the cells of many eukaryotes. T. brucei has no definite shape, it is mobile and has a single flagellum for locomotion.
T. brucei is often found in the rain forests and savannas with tropical climate. It is the cause of the disease trypanosomiasis, also called the African sleeping sickness. Therefore, most cases of trypanosomiasis are found in regions in Western and Central Africa.

Genome structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Does it have any plasmids? Are they important to the organism's lifestyle?

Cell structure and metabolism

T. brucei has organelles including endoplasmic reticulum, golgi apparatus, lysosome, nucleus, and a large mitochondrion. Its mitochondrion houses a unique structure called kinetoplast, where DNA is located. The kinetoplast is associated with the flagellum through microtubules. The organism has a flagellar basal body found posterior to the nucleus. The base of the flagellum is associated with the kinetoplast in the single long mitochondrion. The basal body plays a major role in organizing spindles during mitosis.

Because of its animal-like cells, T. Brucei has heterotrophic cells that require organic molecules for its source of energy. Studies have shown that it possesses some forms of energy producing enzymes. The organism acquires proline as a major energy source when it lives inside the gut of the tsetse fly. Since there is not much nutrients to be obtained from the gut of an insect, the parasite’s mitochondrion needs to carry out some type of metabolic pathways in order to generate sufficient energy. On the contrary, when nutrients become more abundant in mammalian host, the parasite can rely on the glucose from its host as its source of energy without having to undergo metabolic pathways to produce energy for its own use. This causes a severe malnutrition and rapid weight loss in the host.

Ecology

The organism infects mammals by using tsetse flies as intermediate hosts. During a blood meal, the infected Tsetse fly injects the parasite into the host. The organism is carried into the bloodstream of the host through the lymphatic system. T. Brucei begins to replicate in the bloodstream. The parasite migrates to other sites of the body through blood fluids and begins to invade the central nervous system of the host. When a tsetse fly takes a blood meal on the infected host, it becomes infected and goes on to disperse the parasite into the next mammalian host. Also, migration of infected individuals from Africa can spread T. brucei to other regions.

Pathology

Trypanosoma brucei life cycle


How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
The parasitic organism survives in the gut of tsetse flies and then transmitted into the bloodstream of mammalian host and can be carried to the lymph and spinal fluid through circulation.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

Current Research

Enter summaries of the most recent research here--at least three required

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

| Citizendium