Astrocyte: Difference between revisions

From Citizendium
Jump to navigation Jump to search
imported>Pierre-Alain Gouanvic
m (their)
imported>Meg Taylor
No edit summary
 
(15 intermediate revisions by 6 users not shown)
Line 1: Line 1:
{{subpages}}
{{subpages}}
'''Astrocytes''' (also known collectively as '''astroglia''') are characteristic star-shaped [[neuroglia|glial]] [[cell (biology)|cells]] in the [[brain]] and [[spinal cord]].  They perform many functions, including biochemical support of [[endothelial cell|endothelial cells]] which form the [[blood-brain barrier]], the provision of nutrients to the nervous tissue, and a principal role in the repair and scarring process in the brain.
{{TOC|right}}
'''Astrocytes''' (or '''astroglia''') are star-shaped [[neuroglia|glial]] [[cell (biology)|cells]] in the [[brain]] and [[spinal cord]].  They provide metabolic support for the [[epithelial cell]]s that form the [[blood-brain barrier]], provide nutrients to the nervous tissue, and thay have a major role in repair and regeneration in the brain. They also intercommunicate with neurones, and have an important role in the uptake and degradation of neurotransmitters released by neurons into the extracellular space. <ref>Svendsen CN (2002)
Neurobiology: The amazing astrocyte ''Nature'' [http://www.nature.com/nature/journal/v417/n6884/full/417029a.html 417, 29-32]</ref>


==Description==
==Description==
Astrocytes are a sub-type of the [[glial cells]] in the brain and spinal cord. They are also known as ''astrocytic glial cells'', or astroglia. The primary processes of an astrocyte are star-shaped, hence the name; however, the numerous ''fine processes'' largely fill the space between the "star beams" to create a ''spongiform'' shape. Fine distal processes of the astrocyte envelope, or "ensheath" synaptic connections that are formed between [[neuron]]s. Proximity and interaction between synaptic elements and astrocytic processes gave rise to a concept of "tripartite synapse"<ref>{{cite journal |author=Araque A, Parpura V, Sanzgiri RP, Haydon PG |title=Tripartite synapses: glia, the unacknowledged partner |journal=Trends Neurosci. |volume=22 |issue=5 |pages=208–15 |year=1999 |month=May |pmid=10322493 |doi= |url=http://linkinghub.elsevier.com/retrieve/pii/S0166-2236(98)01349-6}}</ref> which is gaining acceptance within the Neuroscience community. The degree of synapse ensheathing varies between brain areas and between individual synapse. Importantly, it also changes over time in an activity-dependent manner, allowing for structural plasticity of tripartite synapse.
Astrocytes are a sub-type of [[glial cells]]. Their primary processes are star-shaped, and numerous fine processes largely fill the space between the "star beams" to create a ''spongiform'' shape. Fine distal processes of the astrocyte envelop, or "ensheath" neurons and their [[axons]]. Proximity and interaction between synaptic elements and astrocytic processes gave rise to a concept of "tripartite synapse"<ref>{{cite journal |author=Araque A ''et al.'' |title=Tripartite synapses: glia, the unacknowledged partner |journal=Trends Neurosci. |volume=22 |pages=208–15 |year=1999 |pmid=10322493 }}</ref>. The degree of synapse ensheathing varies between brain areas and between individual synapses, and it can also change in an activity-dependent manner, allowing for structural plasticity of tripartite synapse. Astrocytes express many different receptors for [[peptides]] and other signalling molecules that are released by neurons, and these signals regulate their shape and function.


Astrocytes are classically identified histologically by their expression of the intermediate filament [[glial fibrillary acidic protein]] (GFAP), although the level of GFAP expression varies widely between individual astrocytes. Two forms of astrocytes exist in the CNS, ''fibrous'' and ''protoplasmic''. Fibrous astrocytes are usually located within white matter, have relatively few organelles, and exhibit long unbranched cellular processes. This type often has "vascular feet", also called endfeet, that physically connect the astrocyte to the outside of capillary wall. Protoplasmic astrocytes, found in grey matter tissue, possess a larger quantity of organelles, and exhibit short and highly branched cellular processes. When in proximity to the [[pia mater]], astrocytes send out their processes to form the pia-glial membrane.
Classically, astrocytes are identified histologically by their expression of the intermediate filament [[glial fibrillary acidic protein]] (GFAP), although the level of GFAP expression varies widely between astrocytes. Two forms of astrocytes exist in the CNS, ''fibrous'' and ''protoplasmic''. Fibrous astrocytes are usually located within white matter, have relatively few organelles, and have long, unbranched processes. This type often has "vascular feet", also called endfeet, that connect the astrocyte to the outside of capillary wall. ''Protoplasmic astrocytes'', found in grey matter, have more organelles, and short,highly branched processes. When in proximity to the [[pia mater]], astrocytes send out their processes to form the pia-glial membrane.


Previously in medical science, the neuronal network was considered the only important one, and astrocytes were looked upon as ''gap fillers''. But recently this view has been reconsidered, and astrocytic networks are now thought to play a number of active roles in the brain, including the secretion and uptake of neurotransmitters, "siphoning" K<sup>+</sup> ions away from synapses, regulation of synaptigenesis, modulation of synaptic transmission, short- and long-term synaptic plasticity, neuro-vascular coupling, maintenance of the blood-brain barrier and more.
Formerly, glia were looked upon as ''gap fillers'' in the CNS, playing essentially a supportive, structural role, and providing neurons with nutrients such as [[glucose]] (the name 'glia' originating from the Greek for glue, and for many years they viewed simply as the brain's packing material)
. However, astrocytic networks are now thought to play a number of active roles in the brain, including the secretion and uptake of neurotransmitters, "siphoning" K<sup>+</sup> ions away from synapses, regulation of [[synaptogenesis]], modulation of [[synaptic transmission]], short- and long-term [[synaptic plasticity]], [[neuro-vascular coupling]], and maintenance of the [[blood-brain barrier]].  


==Functions==
Astrocytes express plasma membrane transporters such as [[glutamate transporter]]s for several neurotransmitters, including [[glutamate]], [[adenosine triphosphate|ATP]]  and [[GABA]]. More recently, astrocytes have been shown shown to release glutamate or ATP in a Ca<sup>2+</sup>-dependent manner.<ref>{{cite journal | author = Santello M, Volterra A | title = Synaptic modulation by astrocytes via Ca(2+)-dependent glutamate release| journal = Neuroscience | year = 2008 | pmid = 18455880 }} </ref><ref>{{cite journal | author = Pryazhnikov E, Khiroug L | title = Synaptic Sub-micromolar increase in [Ca(2+)](i) triggers delayed exocytosis of ATP in cultured astrocytes | journal = Glia| volume = 56:38-49| year = 2008 | pmid = 17910050 }} </ref>
* '''Structural''': involved in the physical structuring of the brain.
* '''Metabolic support''': they provide neurons with nutrients such as [[glucose]].
* '''[[Blood-brain barrier]]''': the astrocyte end-feet encircling [[endothelial cell]]s  were thought to aid in the maintenance of the blood-brain barrier, but recent research indicates that they do not play a substantial role; instead it is the [[tight junctions]] and [[basal lamina]] of the cerebral endothelial cells that play the most substantial role in maintaining the barrier.{{Fact|date=January 2008}}
* '''Transmitter reuptake and release''': astrocytes express plasma membrane transporters such as [[glutamate transporter]]s for several neurotransmitters, including [[glutamate]], ATP and [[GABA]]. More recently, astrocytes were shown to release glutamate or [[adenosine triphosphate|ATP]] in a vesicular, Ca<sup>2+</sup>-dependent manner.{{<ref>{{cite journal | author = Santello M, Volterra A | title = Synaptic modulation by astrocytes via Ca(2+)-dependent glutamate release. | journal = Neuroscience | volume = Mar 22 | [Epub ahead of print] | year = 2008 | pmid = 18455880 | doi = 10.1016/j.neuroscience.2008.03.039}} </ref>}}{{<ref>{{cite journal | author = Pryazhnikov E, Khiroug L. | title = Synaptic Sub-micromolar increase in [Ca(2+)](i) triggers delayed exocytosis of ATP in cultured astrocytes. | journal = Glia. | volume = Jan 1;56(1):38-49. | year = 2008 | pmid = 17910050 | doi = 10.1002/glia.20590}} </ref>}}


* '''Regulation of ion concentration in the extracellular space''': astrocytes express [[potassium channels]] at a high density. When neurons are active, they release potassium, increasing the local extracellular concentration. Because astrocytes are highly permeable to potassium, they rapidly clear the excess accumulation in the extracellular space. If this function is interfered with, the extracellular concentration of potassium will rise, leading to neuronal depolarization by the [[Goldman equation]]. Abnormal accumulation of extracellular potassium is well known to result in epileptic neuronal activity.{{Fact|date=January 2008}}
Astrocytes express a high density of [[potassium channels]]. When neurons are active, they release potassium into the extracellular space; astrocytes can rapidly clear this excess accumulation to maintain the ionic composition of extracellular fluid. If this function is interfered with, the extracellular concentration of potassium will rise, leading to neuronal depolarization , and can result in epileptic neuronal activity.
* '''Modulation of synaptic transmission''': in the [[supraoptic nucleus]] of the [[hypothalamus]], rapid changes in astrocyte morphology have been shown to affect heterosynaptic transmission between neurons.<ref>{{cite journal | author = Piet R, Vargová L, Syková E, Poulain D, Oliet S | title = Physiological contribution of the astrocytic environment of neurons to intersynaptic crosstalk | journal = Proc Natl Acad Sci U S A | volume = 101 | issue = 7 | pages = 2151–5 | year = 2004 | pmid = 14766975 | doi = 10.1073/pnas.0308408100}}</ref>
* '''Vasomodulation''': astrocytes may serve as intermediaries in neuronal regulation of blood flow.<ref>{{cite journal | author = Parri R, Crunelli V | title = An astrocyte bridge from synapse to blood flow | journal = Nat Neurosci | volume = 6 | issue = 1 | pages = 5–6 | year = 2003 | pmid = 12494240 | doi = 10.1038/nn0103-5}}</ref>
* '''Promotion of the myelinating activity of [[oligodendrocyte]]s''': electrical activity in neurons causes them to release ATP, which serves as an important stimulus for myelin to form. Surprisingly, the ATP does not act directly on oligodendrocytes. Instead it causes astrocytes to secrete cytokine leukemia inhibitory factor (LIF), a regulatory protein that promotes the myelinating activity of oligodendrocytes.  This suggest that astrocytes have an executive-coordinating role in the brain.<ref>{{cite journal | author = Ishibashi T, Dakin K, Stevens B, Lee P, Kozlov S, Stewart C, Fields R | title = Astrocytes promote myelination in response to electrical impulses | journal = Neuron | volume = 49 | issue = 6 | pages = 823–32 | year = 2006 | pmid = 16543131 | doi = 10.1016/j.neuron.2006.02.006}}</ref>
* '''Nervous system repair''': upon injury to nerve cells within the central nervous system, astrocytes become phagocytic to ingest the injured nerve cells. The astrocytes then fill up the space to form a [[glial scar]], repairing the area and replacing the CNS cells that cannot regenerate.{{Fact|date=June 2008}}


Recent studies have shown that astrocytes play an important function in the regulation of neural [[stem cell]]s. Research from the Schepens Eye Research Institute at Harvard shows the human brain to abound in neural stem cells, which are kept in a dormant state by  chemical signals (ephrin-A2 and ephrin-A3) from the astrocytes. The astrocytes are able to activate the stem cells to transform into working neurons by dampening the release of [[ephrin-A2]] and [[ephrin-A3]].{{Fact|date=June 2008}}
In the [[supraoptic nucleus]] and [[paraventricular nucleus]] of the [[hypothalamus]], astrocytic processes ensheathe the somata and dendrites of [[oxytocin]]- and [[vasopressin]]- secreting neurons. Rapid changes in the morphology of these astrocytes occur in a variety of different physiological states, including during [[lactation]], which is associated with high demand for oxytocin, and after dehydration, which is associated with high demand for vasopressin. These changes affect dendro-dendritic intercommunication between the neurons, and also affevt afferent synaptic transmission to them, by altering the availability of transmitter released at synapses.<ref>{{cite journal | author = Piet R ''et al.''| title = Physiological contribution of the astrocytic environment of neurons to intersynaptic crosstalk | journal = Proc Natl Acad Sci U S A | volume = 101  | pages = 2151–5 | year = 2004 | pmid = 14766975 }}</ref>
* '''Vasomodulation''': astrocytes may serve as intermediaries in neuronal regulation of blood flow.<ref>{{cite journal | author = Parri R, Crunelli V | title = An astrocyte bridge from synapse to blood flow | journal = Nat Neurosci | volume = 6 | pages = 5–6 | year = 2003 | pmid = 12494240 }}</ref>


Furthermore, studies are underway to determine whether astroglia play an instrumental role in depression, based on the link between diabetes and depression. Altered CNS [[glucose]] metabolism is seen in both these conditions, and the astroglial cells are the only cells with [[insulin]] receptors in the brain.
Electrical activity in neurons causes them to release ATP, which serves as an important stimulus for myelin to form. Surprisingly, ATP does not act directly on oligodendrocytes; instead it causes astrocytes to secrete the cytokine [[leukemia inhibitory factor]], a regulatory protein that promotes the myelinating activity of oligodendrocytes.  <ref>{{cite journal | author = Ishibashi T ''et al.'' | title = Astrocytes promote myelination in response to electrical impulses | journal = Neuron | volume = 49 | pages = 823–32 | year = 2006 | pmid = 16543131 }}</ref>


==Calcium waves==
When nerve cells are injured, astrocytes become phagocytic to ingest them . The astrocytes then fill up the space to form a [[glial scar]], repairing the area and replacing the CNS cells that cannot regenerate.
Astrocytes are linked by [[gap junction]]s, creating an electrically coupled [[syncytium]].<ref>{{cite journal | author = Bennett M, Contreras J, Bukauskas F, Sáez J | title = New roles for astrocytes: gap junction hemichannels have something to communicate | journal = Trends Neurosci | volume = 26 | issue = 11 | pages = 610–7 | year = 2003 | pmid = 14585601 | doi = 10.1016/j.tins.2003.09.008}}</ref>


An increase in intracellular calcium concentration can propagate outwards through this syncytium. Mechanisms of calcium wave propagation include diffusion of [[IP3]] through gap junctions and extracellular ATP signalling.<ref>Newman, J Neurosci. 2001 Apr 1;21(7):2215-23</ref> Calcium elevations are the primary known axis of activation in astrocytes, and are necessary and sufficient for some types of astrocytic glutamate release.<ref>{{cite journal | author = Parpura V, Haydon P | title = Physiological astrocytic calcium levels stimulate glutamate release to modulate adjacent neurons | journal = Proc Natl Acad Sci U S A | volume = 97 | issue = 15 | pages = 8629–34 | year = 2000 | pmid = 10900020 | doi = 10.1073/pnas.97.15.8629}}</ref>
Astrocytes are important in regulating neural [[stem cell]]s. The human brain contains numerous neural [[stem cell]]s, which are kept in a dormant state by  chemical signals (ephrin-A2 and ephrin-A3) from the astrocytes. Astrocytes can activate the stem cells to transform into working neurons by dampening the release of [[ephrin-A2]] and [[ephrin-A3]].


==Calcium waves==
Astrocytes are linked by [[gap junction]]s, creating an electrically coupled [[syncytium]].<ref>{{cite journal | author = Bennett M ''et al.''| title = New roles for astrocytes: gap junction hemichannels have something to communicate | journal = Trends Neurosci | volume = 26 | pages = 610–7 | year = 2003 | pmid = 14585601 }}</ref> Waves of intracellular calcium  can propagate outwards through this syncytium. Mechanisms of calcium wave propagation include diffusion of [[IP3]] through gap junctions and extracellular ATP signalling.<ref>Newman, J (2001) ''Neuroscience'' 21:2215-23</ref> Calcium elevations are the primary known axis of activation in astrocytes, and are necessary and sufficient for some types of astrocytic glutamate release.<ref>{{cite journal | author = Parpura V, Haydon P | title = Physiological astrocytic calcium levels stimulate glutamate release to modulate adjacent neurons | journal = Proc Natl Acad Sci U S A | volume = 97 | pages = 8629–34 | year = 2000 | pmid = 10900020 }}</ref>


==Classification==
==Classification==
There are several different ways to classify astrocytes:
There are several ways to classify astrocytes:
===by Lineage and antigenic phenotype===
These have been established by classic work by Raff et al in early 1980s on Rat optic nerves.
* Type 1: Antigenically Ran2<sup>+</sup>, GFAP<sup>+</sup>, FGFR3<sup>+</sup>, A2B5<sup>-</sup> thus resembling the "type 1 astrocyte" of the postnatal day 7 rat optic nerve. These can arise from the tripotential glial restricted precursor cells (GRP), but not from the bipotential O2A/OPC (oligodendrocyte, type 2 astrocyte precursor, also called ''[[Oligodendrocyte precursor cell|Oligodendrocye progenitor cell]]'') cells.
* Type 2: Antigenically A2B5<sup>+</sup>, GFAP<sup>+</sup>, FGFR3<sup>-</sup>, Ran 2<sup>-</sup>. These cells can develop '''in vitro''' from the either tripotential GRP (probably via O2A stage) or from bipotential O2A cells (which some people think may in turn have been derived from the GRP) '''or''' in vivo when the these progenitor cells are '''transplanted''' into lesion sites (but ''probably not in normal development, at least not in the rat optic nerve''). Type-2 astrocytes are the major astrocytic component in postnatal optic nerve cultures that are generated by O2A cells grown in the presence of fetal calf serum but are not thought to exist in vivo (Fulton et al., 1992).
 
===by Anatomical Classification===
*Protoplasmic: found in [[grey matter]] and have many branching processes whose end-feet envelop [[synapses]]. Some protoplasmic astrocytes are generated by multipotent [[subventricular zone]] progenitor cells.<!--
 
--><ref name="pmid8439409">{{cite journal |author=Levison SW, Goldman JE |title=Both oligodendrocytes and astrocytes develop from progenitors in the subventricular zone of postnatal rat forebrain |journal=[[Neuron]] |volume=10 |issue=2 |pages=201–12 |year=1993 |month=February |pmid=8439409 |doi= |url=http://linkinghub.elsevier.com/retrieve/pii/0896-6273(93)90311-E}}</ref><ref name="pmid7472478">{{cite journal |author=Zerlin M, Levison SW, Goldman JE |title=Early patterns of migration, morphogenesis, and intermediate filament expression of subventricular zone cells in the postnatal rat forebrain |journal=[[J. Neurosci.]] |volume=15 |issue=11 |pages=7238–49 |year=1995 |month=November |pmid=7472478 |doi= |url=http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=7472478}}</ref><!--


-->
'''By lineage and antigenic phenotype'''
*Fibrous: found in [[white matter]] and have long thin unbranched processes whose end-feet envelop [[nodes of Ranvier]].<ref>{{MUNAnatomy|nerve/neuron}}</ref> Some fibrous astrocytes are generated by [[radial glia]].<!--
#Type 1: Antigenically Ran2<sup>+</sup>, GFAP<sup>+</sup>, FGFR3<sup>+</sup>, A2B5<sup>-</sup> thus resembling the "type 1 astrocyte" of the postnatal day 7 rat optic nerve. These can arise from the tripotential glial restricted precursor cells (GRP), but not from the bipotential O2A/OPC (oligodendrocyte, type 2 astrocyte precursor cells).
#Type 2: Antigenically A2B5<sup>+</sup>, GFAP<sup>+</sup>, FGFR3<sup>-</sup>, Ran 2<sup>-</sup>. These cells can develop ''in vitro'' from the either tripotential GRP  or from bipotential O2A cells  '''or''' ''in vivo'' when the these progenitor cells are '''transplanted''' into lesion sites.


--><ref name="pmid77708">{{cite journal |author=Choi BH, Lapham LW |title=Radial glia in the human fetal cerebrum: a combined Golgi, immunofluorescent and electron microscopic study |journal=[[Brain Res.]] |volume=148 |issue=2 |pages=295–311 |year=1978 |month=June |pmid=77708 |doi= |url=http://linkinghub.elsevier.com/retrieve/pii/0006-8993(78)90721-7}}</ref><ref name="pmid111580">{{cite journal |author=Schmechel DE, Rakic P |title=A Golgi study of radial glial cells in developing monkey telencephalon: morphogenesis and transformation into astrocytes |journal=[[Anat. Embryol.]] |volume=156 |issue=2 |pages=115–52 |year=1979 |month=June |pmid=111580 |doi= |url=}}</ref><ref name="pmid3069243">{{cite journal |author=Misson JP, Edwards MA, Yamamoto M, Caviness VS |title=Identification of radial glial cells within the developing murine central nervous system: studies based upon a new immunohistochemical marker |journal=[[Brain Res. Dev. Brain Res.]] |volume=44 |issue=1 |pages=95–108 |year=1988 |month=November |pmid=3069243 |doi= |url=}}</ref><ref name="pmid2808761">{{cite journal |author=Voigt T |title=Development of glial cells in the cerebral wall of ferrets: direct tracing of their transformation from radial glia into astrocytes |journal=[[J. Comp. Neurol.]] |volume=289 |issue=1 |pages=74–88 |year=1989 |month=November |pmid=2808761 |doi=10.1002/cne.902890106 |url=}}</ref><ref name="pmid8844514">{{cite journal |author=Goldman SA, Zukhar A, Barami K, Mikawa T, Niedzwiecki D |title=Ependymal/subependymal zone cells of postnatal and adult songbird brain generate both neurons and nonneuronal siblings in vitro and in vivo |journal=[[J. Neurobiol.]] |volume=30 |issue=4 |pages=505–20 |year=1996 |month=August |pmid=8844514 |doi=10.1002/(SICI)1097-4695(199608)30:4&lt;505::AID-NEU6&gt;3.0.CO;2-7 |url=}}</ref>
''' Anatomically
#Protoplasmic: found in [[grey matter]] and have many branching processes whose end-feet envelop [[synapses]]. Some protoplasmic astrocytes are generated by multipotent [[subventricular zone]] progenitor cells. <ref>{{cite journal |author=Levison SW, Goldman JE |title=Both oligodendrocytes and astrocytes develop from progenitors in the subventricular zone of postnatal rat forebrain |journal=Neuron |volume=10 |pages=201–12 |year=1993 |pmid=8439409 }}</ref><ref>{{cite journal |author=Zerlin M ''et al.'' |title=Early patterns of migration, morphogenesis, and intermediate filament expression of subventricular zone cells in the postnatal rat forebrain |journal=J Neurosci |volume=15 |pages=7238–49 |year=1995 |pmid=7472478}}</ref>
#Fibrous: found in [[white matter]], these have long thin unbranched processes whose end-feet envelop [[nodes of Ranvier]].  Some fibrous astrocytes are generated by [[radial glia]]. <ref>{{cite journal |author=Choi BH, Lapham LW |title=Radial glia in the human fetal cerebrum: a combined Golgi, immunofluorescent and electron microscopic study |journal=Brain Res |volume=148 |pages=295–311 |year=1978 |pmid=77708}}</ref><ref name="pmid111580">{{cite journal |author=Schmechel DE, Rakic P |title=A Golgi study of radial glial cells in developing monkey telencephalon: morphogenesis and transformation into astrocytes |journal=Anat Embryol |volume=156 |pages=115–52 |year=1979 |pmid=111580}}</ref><ref>{{cite journal |author=Misson JP ''et al.''|title=Identification of radial glial cells within the developing murine central nervous system: studies based upon a new immunohistochemical marker |journal=Brain Res |volume=44 |pages=95–108 |year=1988 |pmid=3069243 }}</ref><ref name="pmid2808761">{{cite journal |author=Voigt T |title=Development of glial cells in the cerebral wall of ferrets: direct tracing of their transformation from radial glia into astrocytes |journal=J Comp Neurol |volume=289 |pages=74–88 |year=1989 |pmid=2808761}}</ref><ref>{{cite journal |author=Goldman SA ''et al.'' |title=Ependymal/subependymal zone cells of postnatal and adult songbird brain generate both neurons and nonneuronal siblings in vitro and in vivo |journal=J Neurobiol |volume=30|pages=505–20 |year=1996|pmid=8844514}}</ref>


===by Transporter/receptor classification===
'''By transporter/receptor expression'''
*GluT type: express [[glutamate transporter]]s (EAAT1/{{Gene|SLC1A3}} and EAAT2/{{Gene|SLC1A2}}) and respond to synaptic release of glutamate by transporter currents
#GluT type: express [[glutamate transporter]]s (EAAT1/{{Gene|SLC1A3}} and EAAT2/{{Gene|SLC1A2}})  
*GluR type: express [[glutamate receptors]] (mostly [[Metabotropic glutamate receptor|mGluR]] and [[AMPA receptor|AMPA]] type) and respond to synaptic release of glutamate by channel-mediated currents and [[Inositol triphosphate|IP3]]-dependent Ca2+ transients
#GluR type: express [[glutamate receptors]] (mostly [[Metabotropic glutamate receptor|mGluR]] and [[AMPA receptor|AMPA]] type); these respond to glutamate by channel-mediated currents and IP3-dependent calcium transients


==Bergmann glia==
==Bergmann glia==
''Bergmann glia'', a type of glia<ref>{{cite journal |author=Riquelme R, Miralles C, De Blas A |title=Bergmann glia GABA(A) receptors concentrate on the glial processes that wrap inhibitory synapses |journal=J. Neurosci. |volume=22 |issue=24 |pages=10720–30 |year=2002 |pmid=12486165 |url=http://www.jneurosci.org/cgi/content/abstract/22/24/10720 }}</ref><ref>{{cite journal |author=Yamada K, Watanabe M |title=Cytodifferentiation of Bergmann glia and its relationship with Purkinje cells |journal=Anatomical science international / Japanese Association of Anatomists |volume=77 |issue=2 |pages=94–108 |year=2002 |pmid=12418089}}</ref> also known as radial epithelial cells (as named by [[Camillo Golgi]]), are astrocytes in the [[cerebellum]] that have their [[cell body|cell bodies]] in the [[Purkinje cell]] layer and processes that extend into the molecular layer, terminating with bulbous endfeet at the [[pia mater|pial]] surface. Bergmann glia express high densities of [[glutamate transporter]]s that limit diffusion of the [[neurotransmitter]] [[glutamate]] during its release from synaptic terminals. Besides their role in early development of the cerebellum, Bergmann glia are also required for the pruning or addition of [[synapse]]s.<sup>[[citation needed]]</sup>
''Bergmann glia''<ref>{{cite journal |author=Riquelme R ''et al.'' |title=Bergmann glia GABA(A) receptors concentrate on the glial processes that wrap inhibitory synapses |journal=J. Neurosci |volume=22 |pages=10720–30 |year=2002 |pmid=12486165 |url=http://www.jneurosci.org/cgi/content/abstract/22/24/10720 }}</ref><ref>{{cite journal |author=Yamada K, Watanabe M |title=Cytodifferentiation of Bergmann glia and its relationship with Purkinje cells |journal=Anat Sci Int |volume=77 |pages=94–108 |year=2002 |pmid=12418089}}</ref> also known as radial epithelial cells (as named by [[Camillo Golgi]]), are astrocytes in the [[cerebellum]] that have their [[cell body|cell bodies]] in the [[Purkinje cell]] layer and processes that extend into the molecular layer, terminating with bulbous endfeet at the [[pia mater|pial]] surface. Bergmann glia express high densities of glutamate transporters that limit diffusion of the glutamate during its release from synaptic terminals. Besides their role in early development of the cerebellum, Bergmann glia are also required for the pruning or addition of [[synapse]]s.


==Pathology==
==Pathology==
[[Astrocytoma]]s are primary intracranial [[tumor]]s derived from astrocytes cells of the brain.  
[[Astrocytoma]]s are primary intracranial [[tumor]]s derived from astrocyte cells of the brain. Under the 1993 [[World Health Association]] criteria, a [[glioblastoma]] can be considered a "high-grade" (Grade IV) astrocytoma; grade III anaplastic astrocytomas are also malignant. The lower-grade astrocytomas may progress to higher-grade <ref>{{citation
| title = The new WHO Classification of Tumors affecting the Central Nervous System
| author = Stephen B. Tatter
| year = 2006
| publisher = Neurosurgical Service, [[Massachusetts General Hospital]]}}</ref>


==References==
==References==
{{Reflist|2}}
{{reflist}}

Latest revision as of 06:40, 28 September 2013

This article is developing and not approved.
 Main Article
 Discussion
Related Articles  [?]
Bibliography  [?]
External Links  [?]
Citable Version  [?]
 
https://en.citizendium.org/wiki/index.php?title=Astrocyte&printable=yes
This editable Main Article is under development and subject to a disclaimer.

Astrocytes (or astroglia) are star-shaped glial cells in the brain and spinal cord. They provide metabolic support for the epithelial cells that form the blood-brain barrier, provide nutrients to the nervous tissue, and thay have a major role in repair and regeneration in the brain. They also intercommunicate with neurones, and have an important role in the uptake and degradation of neurotransmitters released by neurons into the extracellular space. [1]

Description

Astrocytes are a sub-type of glial cells. Their primary processes are star-shaped, and numerous fine processes largely fill the space between the "star beams" to create a spongiform shape. Fine distal processes of the astrocyte envelop, or "ensheath" neurons and their axons. Proximity and interaction between synaptic elements and astrocytic processes gave rise to a concept of "tripartite synapse"[2]. The degree of synapse ensheathing varies between brain areas and between individual synapses, and it can also change in an activity-dependent manner, allowing for structural plasticity of tripartite synapse. Astrocytes express many different receptors for peptides and other signalling molecules that are released by neurons, and these signals regulate their shape and function.

Classically, astrocytes are identified histologically by their expression of the intermediate filament glial fibrillary acidic protein (GFAP), although the level of GFAP expression varies widely between astrocytes. Two forms of astrocytes exist in the CNS, fibrous and protoplasmic. Fibrous astrocytes are usually located within white matter, have relatively few organelles, and have long, unbranched processes. This type often has "vascular feet", also called endfeet, that connect the astrocyte to the outside of capillary wall. Protoplasmic astrocytes, found in grey matter, have more organelles, and short,highly branched processes. When in proximity to the pia mater, astrocytes send out their processes to form the pia-glial membrane.

Formerly, glia were looked upon as gap fillers in the CNS, playing essentially a supportive, structural role, and providing neurons with nutrients such as glucose (the name 'glia' originating from the Greek for glue, and for many years they viewed simply as the brain's packing material) . However, astrocytic networks are now thought to play a number of active roles in the brain, including the secretion and uptake of neurotransmitters, "siphoning" K+ ions away from synapses, regulation of synaptogenesis, modulation of synaptic transmission, short- and long-term synaptic plasticity, neuro-vascular coupling, and maintenance of the blood-brain barrier.

Astrocytes express plasma membrane transporters such as glutamate transporters for several neurotransmitters, including glutamate, ATP and GABA. More recently, astrocytes have been shown shown to release glutamate or ATP in a Ca2+-dependent manner.[3][4]

Astrocytes express a high density of potassium channels. When neurons are active, they release potassium into the extracellular space; astrocytes can rapidly clear this excess accumulation to maintain the ionic composition of extracellular fluid. If this function is interfered with, the extracellular concentration of potassium will rise, leading to neuronal depolarization , and can result in epileptic neuronal activity.

In the supraoptic nucleus and paraventricular nucleus of the hypothalamus, astrocytic processes ensheathe the somata and dendrites of oxytocin- and vasopressin- secreting neurons. Rapid changes in the morphology of these astrocytes occur in a variety of different physiological states, including during lactation, which is associated with high demand for oxytocin, and after dehydration, which is associated with high demand for vasopressin. These changes affect dendro-dendritic intercommunication between the neurons, and also affevt afferent synaptic transmission to them, by altering the availability of transmitter released at synapses.[5]

  • Vasomodulation: astrocytes may serve as intermediaries in neuronal regulation of blood flow.[6]

Electrical activity in neurons causes them to release ATP, which serves as an important stimulus for myelin to form. Surprisingly, ATP does not act directly on oligodendrocytes; instead it causes astrocytes to secrete the cytokine leukemia inhibitory factor, a regulatory protein that promotes the myelinating activity of oligodendrocytes. [7]

When nerve cells are injured, astrocytes become phagocytic to ingest them . The astrocytes then fill up the space to form a glial scar, repairing the area and replacing the CNS cells that cannot regenerate.

Astrocytes are important in regulating neural stem cells. The human brain contains numerous neural stem cells, which are kept in a dormant state by chemical signals (ephrin-A2 and ephrin-A3) from the astrocytes. Astrocytes can activate the stem cells to transform into working neurons by dampening the release of ephrin-A2 and ephrin-A3.

Calcium waves

Astrocytes are linked by gap junctions, creating an electrically coupled syncytium.[8] Waves of intracellular calcium can propagate outwards through this syncytium. Mechanisms of calcium wave propagation include diffusion of IP3 through gap junctions and extracellular ATP signalling.[9] Calcium elevations are the primary known axis of activation in astrocytes, and are necessary and sufficient for some types of astrocytic glutamate release.[10]

Classification

There are several ways to classify astrocytes:

By lineage and antigenic phenotype

  1. Type 1: Antigenically Ran2+, GFAP+, FGFR3+, A2B5- thus resembling the "type 1 astrocyte" of the postnatal day 7 rat optic nerve. These can arise from the tripotential glial restricted precursor cells (GRP), but not from the bipotential O2A/OPC (oligodendrocyte, type 2 astrocyte precursor cells).
  2. Type 2: Antigenically A2B5+, GFAP+, FGFR3-, Ran 2-. These cells can develop in vitro from the either tripotential GRP or from bipotential O2A cells or in vivo when the these progenitor cells are transplanted into lesion sites.

Anatomically

  1. Protoplasmic: found in grey matter and have many branching processes whose end-feet envelop synapses. Some protoplasmic astrocytes are generated by multipotent subventricular zone progenitor cells. [11][12]
  2. Fibrous: found in white matter, these have long thin unbranched processes whose end-feet envelop nodes of Ranvier. Some fibrous astrocytes are generated by radial glia. [13][14][15][16][17]

By transporter/receptor expression

  1. GluT type: express glutamate transporters (EAAT1/Template:Gene and EAAT2/Template:Gene)
  2. GluR type: express glutamate receptors (mostly mGluR and AMPA type); these respond to glutamate by channel-mediated currents and IP3-dependent calcium transients

Bergmann glia

Bergmann glia[18][19] also known as radial epithelial cells (as named by Camillo Golgi), are astrocytes in the cerebellum that have their cell bodies in the Purkinje cell layer and processes that extend into the molecular layer, terminating with bulbous endfeet at the pial surface. Bergmann glia express high densities of glutamate transporters that limit diffusion of the glutamate during its release from synaptic terminals. Besides their role in early development of the cerebellum, Bergmann glia are also required for the pruning or addition of synapses.

Pathology

Astrocytomas are primary intracranial tumors derived from astrocyte cells of the brain. Under the 1993 World Health Association criteria, a glioblastoma can be considered a "high-grade" (Grade IV) astrocytoma; grade III anaplastic astrocytomas are also malignant. The lower-grade astrocytomas may progress to higher-grade [20]

References

  1. ↑ Svendsen CN (2002) Neurobiology: The amazing astrocyte Nature 417, 29-32
  2. ↑ Araque A et al. (1999). "Tripartite synapses: glia, the unacknowledged partner". Trends Neurosci. 22: 208–15. PMID 10322493.
  3. ↑ Santello M, Volterra A (2008). "Synaptic modulation by astrocytes via Ca(2+)-dependent glutamate release". Neuroscience. PMID 18455880.
  4. ↑ Pryazhnikov E, Khiroug L (2008). "Synaptic Sub-micromolar increase in [Ca(2+)](i) triggers delayed exocytosis of ATP in cultured astrocytes". Glia 56:38-49. PMID 17910050.
  5. ↑ Piet R et al. (2004). "Physiological contribution of the astrocytic environment of neurons to intersynaptic crosstalk". Proc Natl Acad Sci U S A 101: 2151–5. PMID 14766975.
  6. ↑ Parri R, Crunelli V (2003). "An astrocyte bridge from synapse to blood flow". Nat Neurosci 6: 5–6. PMID 12494240.
  7. ↑ Ishibashi T et al. (2006). "Astrocytes promote myelination in response to electrical impulses". Neuron 49: 823–32. PMID 16543131.
  8. ↑ Bennett M et al. (2003). "New roles for astrocytes: gap junction hemichannels have something to communicate". Trends Neurosci 26: 610–7. PMID 14585601.
  9. ↑ Newman, J (2001) Neuroscience 21:2215-23
  10. ↑ Parpura V, Haydon P (2000). "Physiological astrocytic calcium levels stimulate glutamate release to modulate adjacent neurons". Proc Natl Acad Sci U S A 97: 8629–34. PMID 10900020.
  11. ↑ Levison SW, Goldman JE (1993). "Both oligodendrocytes and astrocytes develop from progenitors in the subventricular zone of postnatal rat forebrain". Neuron 10: 201–12. PMID 8439409.
  12. ↑ Zerlin M et al. (1995). "Early patterns of migration, morphogenesis, and intermediate filament expression of subventricular zone cells in the postnatal rat forebrain". J Neurosci 15: 7238–49. PMID 7472478.
  13. ↑ Choi BH, Lapham LW (1978). "Radial glia in the human fetal cerebrum: a combined Golgi, immunofluorescent and electron microscopic study". Brain Res 148: 295–311. PMID 77708.
  14. ↑ Schmechel DE, Rakic P (1979). "A Golgi study of radial glial cells in developing monkey telencephalon: morphogenesis and transformation into astrocytes". Anat Embryol 156: 115–52. PMID 111580.
  15. ↑ Misson JP et al. (1988). "Identification of radial glial cells within the developing murine central nervous system: studies based upon a new immunohistochemical marker". Brain Res 44: 95–108. PMID 3069243.
  16. ↑ Voigt T (1989). "Development of glial cells in the cerebral wall of ferrets: direct tracing of their transformation from radial glia into astrocytes". J Comp Neurol 289: 74–88. PMID 2808761.
  17. ↑ Goldman SA et al. (1996). "Ependymal/subependymal zone cells of postnatal and adult songbird brain generate both neurons and nonneuronal siblings in vitro and in vivo". J Neurobiol 30: 505–20. PMID 8844514.
  18. ↑ Riquelme R et al. (2002). "Bergmann glia GABA(A) receptors concentrate on the glial processes that wrap inhibitory synapses". J. Neurosci 22: 10720–30. PMID 12486165.
  19. ↑ Yamada K, Watanabe M (2002). "Cytodifferentiation of Bergmann glia and its relationship with Purkinje cells". Anat Sci Int 77: 94–108. PMID 12418089.
  20. ↑ Stephen B. Tatter (2006), The new WHO Classification of Tumors affecting the Central Nervous System, Neurosurgical Service, Massachusetts General Hospital
Retrieved from "https://citizendium.org/wiki/index.php?title=Astrocyte&oldid=530379"