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'''''[[Clostridium difficile]]''''' is a spore-forming, anaerobic, toxin-producing bacterium that is a "common inhabitant of the colon flora in human infants and sometimes in adults. It produces a toxin that causes pseudomembranous enterocolitis in patients receiving antibiotic therapy." ''C. difficile'' superinfection after oral antibiotic therapy, leading to potentially fatal pseudomembranous enterocolitis, has been an increasingly severe public health problem. Indeed, many primary physicians now consider it wise to warn outpatients on antibiotics to seek immediate consultation if they develop severe diarrhea.
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C. difficile is present at low levels in the gut flora of about 3% of adults. These people however show no symptoms and do not need to be treated. The infection occurs when a person is treated with antibiotics targeted against other bacteria. The disease is for the most part nosocomial. Patients who are hospitalized come in contact and are often inoculated with the bacteria. When the patient is treated with antibiotics, especially those with a broad range of activity, the normal gut flora is disrupted, and C. difficile, with its multi-drug resistance, experiences overgrowth. The bacteria releases large quantities of enterotoxins (toxin A) and cytotoxins (toxin B), causing pseudomembranous enterocolitis.
==Footnotes==
 
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[[Image:img2.gif|thumb|left|250px|scanning electron micrograph of C. difficile]]
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==History==
In 1935, Hall and O’Toole first isolated the bacteria from the stools of newborns and described it. They named it ''Bacillus difficilis'' because it was hard to isolate and grew very slowly in culture.
 
C. difficile is an important pathogen that is currently increasing in its prevalence world-wide. A complete genome sequence would enable geneticists to come up with a more direct and efficient treatment against the pathogen. The genetic material encodes for antimicrobial resistance, production of toxins (virulence), host interaction (adaptations for survival and growth within the gut environment), and the production of surface structures. The understanding of how these genes interact with their environment will be useful in developing therapies against C. difficile associated diseases.
 
==Genome Structure==
Sebaihia et al (2006) determined the complete genomic sequence of ''C. difficile'' strain 630, a highly virulent and multidrug-resistant strain. It was found that the genome consists of a circular chromosome of 4,290,252 bp and a plasmid, pCD630, of 7,881 bp. The chromosome encodes 3,776 predicted coding sequences (CDSs), with resistance, virulence, and host interaction genes, while the plasmid carries only 11 CDSs, none of which has any obvious function. ''C. difficile'' has a highly mobile genome, with 11% of the genome consisting of mobile genetic elements, mostly in the form of conjugative transposons. Conjugative transposons are mobile genetic elements that are capable of integrating into and excising from the host genome and transferring themselves, and are responsible for the evolutionary acquisition by C. difficile of genes involved in resistance, virulence, and host interactions. Some of the mobile elements are prophage sequences. Host interaction genes involve genes that code for metabolic capability adaptations for survival and growth within the gut environment.
 
''[[Clostridium difficile|.... (read more)]]''

Latest revision as of 10:19, 11 September 2020

Nuclear weapons proliferation is one of the four big issues that have held back worldwide deployment of peaceful nuclear power. This article will address the proliferation questions raised in Nuclear power reconsidered.

As of 2022, countries with nuclear weapons have followed one or both of two paths in producing fissile materials for nuclear weapons: enrichment of uranium to very high fractions of U-235, or extraction of fissile plutonium (Pu-239) from irradiated uranium nuclear reactor fuel. The US forged the way on both paths during its World War II Manhattan Project. The fundamental aspects of both paths are well understood, but both are technically challenging. Even relatively poor countries can be successful if they have sufficient motivation, financial investment, and, in some cases, direct or illicit assistance from more technologically advanced countries.

The International Non-proliferation Regime

The International Atomic Energy Agency (IAEA) has a vigorous program to prevent additional countries from acquiring nuclear weapons. The Treaty on the Non-Proliferation of Nuclear Weapons (NPT) is the cornerstone arrangement under which strategic rivals can trust, by independent international verification, that their rivals are not developing a nuclear weapons threat. The large expense of weapons programs makes it very unlikely that a country would start its own nuclear weapons program, if it knows that its rivals are not so engaged. With some notable and worrying exceptions, this program has been largely successful.

Paths to the Bomb

It is frequently claimed that building a civil nuclear power program adds to the weapons proliferation risk. There is an overlap in the two distinct technologies, after all. To build a bomb, one needs Highly Enriched Uranium (HEU) or weapons-grade plutonium (Pu-239). Existing reactors running on Low Enriched Uranium (LEU, under 5% U-235) or advanced reactors running on High Assay LEU (HALEU,up to 20% U-235) use the same technology that can enrich uranium to very high levels, but configured differently. Enrichment levels and centrifuge configurations can be monitored using remote cameras, on-site inspections, and installed instrumentation -- hence the value of international inspections by the IAEA. Using commercial power reactors as a weapons plutonium source is an extremely ineffective, slow, expensive, and easily detectable way to produce Pu. Besides the nuclear physics issues, refueling pressurized water reactors is both time-consuming and obvious to outside observers. That is why the US and other countries developed specialized Pu production reactors and/or uranium enrichment to produce fissile cores for nuclear weapons.

Future Threats and Barriers

Minimizing the risk of future proliferation in states that want to buy nuclear reactors or fuel might require one or more barriers:
1) Insisting on full transparency for all nuclear activities in buyer states, including monitoring and inspections by the International Atomic Energy Agency (IAEA).
2) Limiting fuel processing to just a few supplier states that already have weapons or are approved by the IAEA.
3) Ensuring that fuel at any stage after initial fabrication has an isotopic composition unsuitable for weapons. "Spiking" the initial fuel with non-fissile isotopes, if necessary.
4) Limiting the types of reactors deployed to buyer states. In general, breeders are less secure than burners. Sealed reactor modules are more secure than reactors with on-site fuel processing.
5) Providing incentives and assurances for buyer states to go along with all of the above.
6) Application of diplomatic pressure, sanctions, and other economic measures to non-compliant states.
7) Agreement that any reactor declared rogue by the IAEA will be "fair game" for any state feeling threatened.

Footnotes