Listeria monocytogenes

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Listeria monocytogenes
Listeria monocytogenes.jpg
Scientific classification
Kingdom: Bacteria
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Listeriaceae
Genus: Listeria
Species: monocytogenes
Binomial name
Listeria monocytogenes

Description and significance

Listeria monocytogenes is a gram positive, rod shaped bacterium that is usually arranged in short chains. When smeared L. monocytogenes may be coccoid and mistaken for Streptococci and larger cells tend to resemble corynebacteria. Flagella are present at room temperature. Are motile due to flagella and can also use obtain motility by using the host cell actin filaments to move within and between cells. [1] The bacterium L. monocytogenesis nearly found everywhere in the environment and can be isolated from wild and domestic animals, birds, insects, soil, wastewater, and vegetation. L. monocytogenes are able to survive in a wide range of varying temperatures from below freezing to body temperature and typically grows best at 0 degrees Fahrenheit to 50 degrees Fahrenheit. In fact this very range of temperature is used for freezing and refrigeration! It is resistant to freezing, drying, and heat. Which is extremely well for a bacterium that does not form spores. [2]


Listeria monocytogenes.

L. monocytogenes is an intracellular pathogen that is the cause of the major food born infection Listeriosis. The disease is transmitted from the consumption of mainly contaminated dairy and meat products. L. monocytogenes is carried in the intestinal tract and 5-10% of the human population who carry no apparent symptoms of the disease. [3] The individuals that are highly susceptible to this disease are pregnant women, the elderly, and individuals with deficient immunity due to illness or medication. [4]


In the United States an estimated 2,500 persons become seriously ill with Listeriosis and 500 of these individuals die. [5]

Sequencing the genome of this deadly bacterium is vital as a basis for improving food safety and is one that is most highly researched. By using comparative analysis and gene arrays researchers are able to study the traits of evolution and new traits.

The bacterium Listeria monocytogenes is named after Joseph Lister who introduced sterilization to the realm of medicine. [6]

Since the 1960s the bacterium L.monocytogenes was thought to only cause infection in animals. It was first discovered ninety years ago in rabbits and pigs. It's lethal effects as a foodborne pathogen wasn't apparent until 1981 after a major outbreak. [7] However, it soon came to light that both animals and humans carry this disease and that it can be isolated from numerous sources in the environment.

Genome structure

Listeria Monocytogenes has two strains of sequences that have been sequenced. Several others are currently being sequenced. The size of these strains is 2.9 Mb and the percentage content of %GC is 37- 39%. [8]. The only two strains that have been sequenced are L. monocytogenes strain EGD-e (serotype 1/2a) and Listeria innocua CLIP 11262 (serotype 6a). The strand EGC-e is 2,944,523 base pairs long with 2,853 open reading frames and a %GC percent content of 39%.[9] One of the currently researched strands is F236 (serotype 4b, cheese isolate) and it is 2,905,187 base pairs long. The percent content of AT is 62.0%. The number of genes present in this strain is 2, 821 genes, 1,030 base pairs per gene.[10] About 50% of the sporadic Listeria infection outbreaks have been determined to be from the strain F236 serotype 4b. [11].


Listeria monocytogenes has a single circular chromosome. The ability for Listeria Monocytogenes to be able to survive in a wide range of environments is made possible by the 331 genes encoding different transport proteins. [12]

In a recent study, the plasmid content of 30 isolates of Listeria monocytogenes was obtained from short-ripened cheeses and further analyzed. “The isolates of L. monocytogenes seagroup harbored a single plasmid, pLM33 (33.2 kbp).” [13]

Cell structure and metabolism

Listeria Monocytogenes is a Gram positive bacterium. Like several other gram positive bacterium many of its surface proteins are harbored to the cell wall by a mechanism requiring a COOH-terminal sorting signal with a conserved LPXTG motif. [14].

Listeria Monocytogenes is a pathogenic bacteria that invades human epithelial cells. It is motile due to its flagellum and can move within eukaryotic cells by explosive polymerization of actin filaments. [15]. "It's flagellum strucutures allows motility and by far improves L. monocytogenes infectivity soon after bacterial ingestion in vivo." [16]

Listeria Monocytogenes is a aerobic, facultative intracellular parasite and is non-spore forming. It is different from other gram-positive bacteria in that it produces a endotoxiin. It is often difficult to diagnose Listeria Monocytogenes and certain tests are normally run to test it. Such tests must be positive to conclude that the bacterium is Listeria Monocytogenes. These tests are gram stain, catalase, hydrolysis of sodium hippurate and esculin, motility, alpha-hemolysis and it must test negative for H2S. Listeria Monocytogenes is dependent upon its host to disperse and has little to no motility in a human host. [17]

This bacterium is able to utilize glucose-1-phosphate in a PrfA-dependent way. It can also use glucose-, fructose-, and mannose-6-phosphate efficiently for energy source. The other way it acquires energy is through mediating the import of glucose-6-phosphate and other such sugars from the cytoplasm of the infected cell into the bacterium. Thereon, these sugars can be used as carbon and such energy sources that are important for bacterial survival and for rapid repliction within the host cell. [18]

Ecology

Listeria Monocytogenes is found nearly everywhere in the environment. This bacterium has been found in at least 37 mammalian species. [19] It is very dependent upon its host for disperal. This bacterium has also been known to be found in fecal matter and the soil. It is also a food-borne pathogen that affects both animals and humans. The symptoms have been known to range from mild to severe death. This bacterium is sufficient in surviving a ranging wide of various environmental conditions. Such harsh environmental conditions include high salt concentrations, high pH, and high temperature. Another known interesting characterisic of this bacterium is that it can form biofilms which allow it to attach to a solid surface as a community. From thereon they proliferate and their removal is difficult. [20] In all its existence is parasitic since it is known to cause illness to its host in specific cases. It has been found that a strain of this bacterium is found in healthy individuals who experience no harmful effect from its presence.




Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.

Pathology

Listeria Monocytogenes is an intracellular pathogen of both humans and animals. It is the cause of the food born infection Listeriosis. Outbreaks of the disease are found to be caused by consumption of contaminated milk, soft cheese, and meat products. Symptoms include gastrointestinal symptoms of vomiting and diarrhea as well as patient symptoms of influenza. Individuals that are more highly susceptible of acquiring the infection Listeriosis are pregnant women and people who are immune-suppressed due to illness or medication. For one, the reason that there is such an increased risk in pregnant women is that it may be related to both systematic and local immunological changes associated with pregnancy. In immune-suppressed individuals the invasion, intracellular multiplication, and cell to cell spread of bacterium appears to be mediated through proteins such as internalin, the hemolysin O, listeriolysin, and phospholipase C. Also bear in mind that since Listeria Monocytogenes is an intracellular pathogen it will affect immune-suppressed individuals more.

Unlike the several other food-borne pathogens which are the cause of gastrointestinal illness, L. monocytogenes is causes serious disease such as meningitis, sepsis, encephalitis, corneal ulcer, pneumonia, and stillbirth. The diagnosis of Listeria is by the culture of the bacterium. Also, the treatment method of this disease is intravenous administration of either ampicillin or penicillin.

However, it must be noted that L. monocytogenes is found to be part of the normal gastrointestinal microbiota in healthy individuals resulting in no symptoms. The effect of the pathogen depends not only on the individual but also the strain of bacterium.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

Current Research

Enter summaries of the most recent research here--at least three required

References

  1. ↑ "Listeria Monocytogenes." Wikipedia, The Free Encylopedia. 4 April 2008.[1]
  2. ↑ "A focus on Listeria Monocytogenes." USDA: National Agricultural Library. 4 April 2008. [2]
  3. ↑ "Listeria monocytogenes." Foodborne Pathogenic Microorganisms and Natural Toxins Handbook. U.S. Food and Drug Administration. 4 April 2008. [3]
  4. ↑ "Listeria monocytogenes and Listeriosis." Todar's Online Textbook of Bacteriology. 4 April 2008. [4]
  5. ↑ "Division of Foodborne, Bacterial and Mycotic Diseases (DFBMD). CDC. 4 April 2008. [5]
  6. ↑ "Listeria Monocytogenes." Wikipedia, The Free Encylopedia. 4 April 2008.[6]
  7. ↑ "A focus on Listeria Monocytogenes." USDA: National Agricultural Library. 4 April 2008. [7]
  8. ↑ Willey, Sherwood, and Woolverton. Prescott, Harley, and Klein’s Microbiology, Seventh Edition. McGraw-Hill Companies: New York, 2008. 386-387.
  9. ↑ "Listeria Monocytogenes." Mircobe Wiki. 4 April 2008. [8]
  10. ↑ Peter F. Hallin and David Ussery (2004). CBS Genome Atlas Database: A dynamic storage for bioinformatic results and sequence data Bioinformatics 20:3682-3686 PDF | Pubmed
  11. ↑ "A focus on Listeria Monocytogenes." USDA: National Agricultural Library. 4 April 2008. [9]
  12. ↑ "Listeria Monocytogenes." Mircobe Wiki. 4 April 2008. [10]
  13. ↑ “Characterization of plasmids from Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheeses.” Science Direct. [11]
  14. ↑ “Anchor structure of cell wall surface proteins in Listeria monocytogenes.” Pubmed. [12]
  15. ↑ "Listeria Monocytogenes." Wikipedia, The Free Encylopedia. 4 April 2008. [13]
  16. ↑ "Listeria monocytogenes Flagella Are Used for Motility, Not as Adhesins, To Increase Host Cell Invasion." Infection and Immunity. [14].
  17. ↑ "Listeria Monocytogenes." Microbiology and Bacteriology. [15]
  18. ↑ "Hpt, a bacterial homolog of the microsomal glucose-6-phosphate translocase, mediates rapid intracellular proliferation in Listeria." Genome Biology. [16]
  19. ↑ "Listeria Monocytogenes." U.S. Food and Drug Administration. 5 April 2008. [17]
  20. ↑ "Listeria Monocytogenes." Microbe Wiki. 5 April 2008 [18]

1. "Listeria Monocytogenes." Wikipedia, The Free Encylopedia. 4 April 2008. [19]

2. "Listeria monocytogenes." Foodborne Pathogenic Microorganisms and Natural Toxins Handbook. U.S. Food and Drug Administration. 4 April 2008. [20]

3. "Division of Foodborne, Bacterial and Mycotic Diseases (DFBMD). CDC. 4 April 2008. [21]

4. "A focus on Listeria Monocytogenes." USDA: National Agricultural Library. 4 April 2008. [22]

5. "Incidence of Food Borne Illness 1996-2005: Listeria*." The Case of the Cold-Loving Microbe. 4 April 2008. [23]

6. "Listeria monocytogenes and Listeriosis." Todar's Online Textbook of Bacteriology. 4 April 2008. [24]

7. "Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Pubmed. 4 April 2008. [25]

8. Peter F. Hallin and David Ussery (2004). CBS Genome Atlas Database: A dynamic storage for bioinformatic results and sequence data Bioinformatics 20:3682-3686 PDF | Pubmed

9. "Listeria Monocytogenes." The Institute For Genomic Research. 4 April 2008. [26]

10. " Whole-Genome Sequence of Listeria welshimeri Reveals Common Steps in Genome Reduction with Listeria innocua as Compared to Listeria monocytogenes." Journal of Bacteriology, November 2006, p. 7405-7415, Vol. 188, No. 21. [27]

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