Restriction enzyme: Difference between revisions
Jump to navigation
Jump to search
imported>Todd Coles No edit summary |
imported>Chris Day No edit summary |
||
| Line 1: | Line 1: | ||
{{subpages}} | {{subpages}} | ||
DNA '''restriction enzymes''' (restriction endonucleases) are [[proteins]] that cut [[DNA]] at specific sequences. Each restriction enzyme is specific to a small (commonly 4-6 base pair) sequence. These enzymes can be used to cut DNA into fragments in a specific and repeatable fashion. Uses include preparation of DNA for procedures such as a [[Southern blot]]. | DNA '''restriction enzymes''' (restriction endonucleases) are [[proteins]] that cut [[DNA]] at specific sequences. Each restriction enzyme is specific to a small (commonly 4-6 base pair) sequence. These enzymes can be used to cut DNA into fragments in a specific and repeatable fashion. Uses include preparation of DNA for procedures such as a [[Southern blot]]ting or subcloning into [[plasmid vectors]]. | ||
Revision as of 13:36, 16 February 2009
DNA restriction enzymes (restriction endonucleases) are proteins that cut DNA at specific sequences. Each restriction enzyme is specific to a small (commonly 4-6 base pair) sequence. These enzymes can be used to cut DNA into fragments in a specific and repeatable fashion. Uses include preparation of DNA for procedures such as a Southern blotting or subcloning into plasmid vectors.